- Synonyms
-
- Source
- Yeast
- Molecular Weight
- Approximately 19.9 kDa, a single glycosylated polypeptide chain containing 172 amino acids.
- AA Sequence
- CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ EMVEGDQLQK DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT LLEQLCTGLQ QQLDHLDTCR GQVMGEEDSE LGNMDPIVTV KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR ALTVSTTLQK RLTKMGGDLN SP
- Purity
- > 97 % by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral resistance assay is no less than 1.0 × 107 IU/mg.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
- Endotoxin
- Less than 0.1 EU/µg of rOvIFN-τ as determined by LAL method.
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Usage
- This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
- SDS-PAGE
![](/cnuploadfiles/SDS/166-07 rOvIFN-tau.png)
- Reference
- 1. Clayette P, Martin M, Dereuddre-Bosquet N, et al. 1999. Pathol Biol (Paris), 47: 553-9.
2. Tekin S, Ealy AD, Wang SZ, et al. 2000. J Interferon Cytokine Res, 20: 1001-5.
3. Tennakoon DK, Smith R, Stewart MD, et al. 2001. J Interferon Cytokine Res, 21: 785-92.
4. Ezashi TandRoberts RM. 2004. Endocrinology, 145: 4452-60.
5. Asselin E, Lacroix D, Fortier MA. 1997. Mol Cell Endocrinol, 132: 117-26.
- Background
- IFN-τ is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN-τ has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-α/β. The current investigation concerns the effect of recombinant ovine IFN-tau (rOvIFN-τ) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and up regulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS is via interference with IFN-γ-induced MHC class II expression. IFN-τwas shown to down regulate IFN-γ-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in down regulating inflammation in the central nervous system (CNS). IFN-τdid not upregulate the expression of MHC class II on CVE. IFN-τalso inhibited the replication of Theiler's virus in CVE.