- Synonyms
- HBEGF, DT-R
- Source
- Escherichia coli
- Molecular Weight
- Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
- Size
- 10 µg/100 µg/500 µg
- Sequence
- DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
- Purity
- > 97% by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 U/mg.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in 10 mM PB, 500 mM NaCl, 5% trehalose, pH 7.4.
- Endotoxin
- Less than 0.1 EU/µg of rHuHB-EGF as determined by LAL method.
- Reconstitution
- Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/ml. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Shipping
- The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Usage
- This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
- SDS-PAGE
- Reference
- 1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.
2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.
- Background
- Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73% and 76% a.a. sequence identity with murine and rat HB-EGF.