- Synonyms
- Za11
- Source
- Escherichia coli.
- Molecular Weight
- Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
- AA Sequence
- QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS
- Purity
- > 98 % by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 × 105 U/mg.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
- Endotoxin
- Less than 0.01 EU/µg of rHuIL-21 GMP as determined by LAL method.
- Sterility
- Negative.
- Mycoplasma
- Negative when tested by RD-RCA.
- Host Cell Protein
- Less than 0.05 % when tested by ELISA.
- Host Cell DNA
- Less than 20 ng/mg when tested by qPCR.
- In vitro virus assay
- Negative.
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.5 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Shipping
- The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Usage
- This GMP product can be for research use or further manufacturing use.
- Quality statement
- The manufacture and testing of this product is in compliance with ICH Q7a guidelines.
- Reference
- 1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43.
2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91.
3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264.
4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.
- Background
- Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies.