性能可靠:

• ◆ 高结合力，低脱落率
• ◆ 降低运作成本
• ◆ 无动物源组分发酵过程
• ◆生产过程符合ISO9001:2015质量体系认证

• ◆ 稳定供应

性能指标: 

• ◆ 配基：从大肠杆菌中表达的重组葡萄球菌蛋白A(rSPA)包含5个抗体结合区域(E,D,A,B,C)
• ◆ 凝胶结构：4%交联的琼脂糖凝胶
• ◆ 凝胶粒径：45-165 μm
• ◆ 平均凝胶粒径：90 μm
• ◆配基含量：每ml介质含有约5mg蛋白
• ◆ 结合力：＞30mg人IgG/ml介质
• ◆ 蛋白A脱落率：＜10ng/mg IgG
• ◆ 碱性稳定性：0.1M NaOH，100个循环
• ◆推荐流速：20-150 cm/h
• ◆ 长期3-9，短期2-10

 Protein A 琼脂糖凝胶树脂（瓶装） 

Cat. #	Product Name	Size A	Size B	Size C	Size D
110107A	Protein A ResinCL-4B	5ml	25mg	200ml	1000ml

Protein A 琼脂糖凝胶预装柱

Cat. #	Product Name	Size
120107A1B	Protein A Separose CL-4B Column, 1ml, 2 columns	2x1ml
120107A1E	Protein A Separose CL-4B Column, 1ml, 5 columns	5x1ml
120107A5A	Protein A Separose CL-4B Column, 5ml, 1 column	1x5ml
120107A5E	Protein A Separose CL-4B Column, 5ml, 5 columns	5x5ml

 

抗体纯化介质
---Protein A, Protein G, Protein L, Protein A/G, Protein A/G/L

 

优势
• ◆ 产品种类齐全
• ◆ 无动物源组分
• ◆ 高纯度：＞97%
• ◆ 生产过程符合ISO9001:2008质量体系认证
• ◆ 批次之间稳定性高，低内毒素（＜0.1EU/μg）

• ◆ 稳定供应

 

Protein A

从大肠杆菌中表达的蛋白A与天然的蛋白A在功能上接近，它包含5个IgG抗体结合区域（E-D-A-B-C），结合免疫球蛋白Fc片段，是纯化单克隆和多克隆IgG抗体的理想方法。

Cat. #	名称	描述	规格 A	规格 B	规格 C	定制
1001-01	Protein A,His	Protein A with His-tag at N-terminus	10mg	100mg	1g	Inquiry
1001-02	Protein A-Cys, His	Protein A with His-tag at N-terminus, Cys residue at C-terminus	10mg	100mg	1g	Inquiry
1001-04	Protein A-Cys	Protein A with Cys residue at C-terminus	10mg	100mg	1g	Inquiry
1001-05	Protein A	5 IgG-binding domains	10mg	100mg	1g	Inquiry
1001-07	Protein A,Long Form	5 IgG-binding domains and cell membrane binding region,similar to native Protein A	10mg	100mg	1g	Inquiry
1001-09	Protein A-Cys,Long Form	5 IgG-binding domains and cell membrane binding region,similar to native Protein A,Cys residue at C-terminus	10mg	100mg	1g	Inquiry

Protein G


蛋白G是从溶血性链球菌中分离出来的胞壁蛋白，对不同种属同一类型或不同亚型的免疫球蛋白Fc段均有高度亲和能力。通过基因工程重组的蛋白G包含3个免疫球蛋白结合区域，能够与来自人、小鼠、大鼠、豚鼠、兔、山羊、绵羊、马的IgG结合。而且，同样可以结合Fab段，为IgG的Fab片段纯化提供方便。

Cat. #	名称	描述	规格 A	规格 B	规格 C	定制
1002-01	Protein G-His	Contains 3 immunoglobulin -binding regions with His-tag at C-terminus	1mg	10mg	1g	Inquiry
1002-03	Protein G	Contains 3 immunoglobulin -binding regions	1mg	10mg	1g	Inquiry
1002-04	Cys-Protein G	Contains 3 immunoglobulin -binding regions with Cys residus at N-terminus	1mg	10mg	1g	inquiry

Protein A/G

以基因工程方法重组的protein A/G 包含Protein A的5个免疫球蛋白结合区域和Protein G 的2个结合区域，结合能力较单一的Protein A和Protein G有很大提高。更强的Fc段结合能力使之成为免疫球蛋白纯化更受青睐的工具。protein A/G结合人的所有IgG亚型和IgA、IgE、IgM及少量的IgD。

Cat. #	名称	描述	规格A	规格 B	规格 C	定制
1003-01	Protein A/G-His	Contains 7 immunoglobulin -binding regions with His-tag at C-terminus	1mg	5mg	1g	Inquiry
1003-02	Protein A/G	Contains 7 immunoglobulin -binding regions	1mg	5mg	1g	Inquiry
1003-03	Cys-Protein A/G	Contains 7 immunoglobulin -binding regions with Cys residus at N-terminus	1mg	10mg	1g	Inquiry
1003-04	Protein A/G-Cys	Contains 7 immunoglobulin -binding regions with Cys residus at C-terminus	1mg	5mg	1g	Inquiry

Protein L

Protein L包含5个kappa结合区域，可在不影响抗原结合的情况下与免疫球蛋白κ轻链结合,相较于其它抗体结合蛋白，Protein L具有更广的Ig和Ig亚型结合范围。

Cat. #	名称	描述	规格 A	规格 B	规格 C	定制
1004-01	Protein L-His	Contains 5 immunoglobulin -binding regions with His-tag at N-terminus	1mg	10mg	1g	Inquiry
1004-02	Protein L-Cys,His	Contains 5 immunoglobulin -binding regions with His-tag at N-terminus and Cys residus at C-terminus	1mg	10mg	1g	inquiry
1004-03	Protein L-Cys	Contains 5 immunoglobulin -binding regions with Cys residus at C-terminus	1mg	10mg	1g	inquiry
1004-04	Protein L	Contains 5 immunoglobulin -binding regions	1mg	10mg	1g	inquiry
1004-05	Cys-Protein L	Contains 5 immunoglobulin -binding regions with Cys residus at N-terminus	1mg	10mg	1g	inquiry

Protein A/G/L

Protein A/G/L包含Protein L的5个抗体结合区域，Protein A的5个抗体结合区域和Protein G的2个抗体结合区域。可结合人所有IgG亚型和IgA、IgE、IgM及IgD。

Cat. #	名称	描述	规格 A	规格 B	规格 C	定制
1006-01	Protein A/G/L	Contains 12 immunoglobulin -binding regions	1mg	5mg	1g	inquiry
1006-02	Protein A/G/L-Cys	Contains 12 immunoglobulin -binding regions with Cys residus at C-terminus	1mg	5mg	1g	inquiry
1006-03	Cys-Protein A/G/L	Contains 12 immunoglobulin -binding regions with Cys residus at N-terminus	1mg	10mg	1g	inquiry

结合力综览表

		Protein A	Protein G	Protein L	Protein A/G	Protein A/G/L
	IgG	+++	+++	+++	+++	+++
	IgG1	++++	++++	++++	++++	++++
	IgG2	++++	++++	++++	++++	++++
	Ig3		+++	+++	+++	+++
	IgG4	++++	++++	++++	++++	++++
	IgA	+		+++	+	++++
	IgA1	+		+++	+	++++
	IgA2	+		+++	+	++++
	IgD			+++		++++
	IgE	++		+++	++	++++
	IgM	+		+++	+	++++
	Fab	+	+	+++	+	++++
	ScFv	+		+++	+	++++
Mouse	IgG	++	++	+++	+++	++++
	IgG1	+	++++	+++	+++	++++
	IgG2a	++++	++++	+++	++++	++++
	IgG2b	+++	+++	+++	+++	+++
	IgG3	++	+++	+++	+++	+++
	IgM			+++		+++
Rat	IgG	+	++	+++	++	+++
	IgG1		+	+++	++	+++
	IgG2a		++++	+++	+++	++++
	IgG2b		++	+++	+	+++
	IgG2c	++	++	+++	+++	+++
	IgG3	+	++	?	++	++
Horse	IgG	++	++++	?	++++	++++
	IgG(ab)	+		?	+	+
	IgG(c)	+		?	+	+
	IgG(T)		+++	?	+++	+++
Goat	IgG	++	+++		++++	++++
	IgG1	+	+++		+++	+++
	IgG2	+++	+++		+++	+++
Guinea-pig	IgG	+++	+	?	+++	+++
	IgG1	++	+	?	++	++
	IgG2	++	+	?	++	++
Sheep	IgG	+	++		+++	+++
	IgG1	+	++		+++	+++
	IgG2	+++	+++		+++	+++
Cow	IgG	+	+++		+++	+++
	IgG1	+	+++		+++	+++
	IgG1	++	+++		+++	+++
Rabbit	IgG	+++	+++	+	+++	+++
Cat	IgG	+++	+	?	+	+++
Dog	IgG	++	+	?	+++	+++
Pig	IgG	+++	++	+++	+++	+++
Chicken	IgY
Monkey	IgG	++++	++++	?	++++	++++
Hamster	IgG	+	++	+++	++	++
Koala	IgG		+	?	+	+
Llama	IgG		+	?	+	+
Donkey	IgG	++	+++	?	+++	+++

+: 结合力, ?: 未测试

For more products>>