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货号:104-09
Recombinant Human Fibroblast Growth Factor 9
rHuFGF-9
技术参数
SynonymsGAF, Glia-activating factor, HBGF-9
AccessionP31371
GeneID2254
Source Escherichia coli.
Molecular Weight Approximately 23.3 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids.
Quantity 5µg/20µg/1000µg
AA Sequence APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/µg of rHuFGF-9 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 1 × PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
SDS-PAGE
Reference1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.
2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.
3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
BackgroundFibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The human FGF-9 shares 98 % a.a. sequence identity with mouse, rat, equine, porcine, and bovine FGF-9.