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货号:101-19
Recombinant Human Interleukin-19
rHuIL-19
技术参数
SynonymsMelanoma Differentiation-associated Protein-like Protein, NG.1
Species9
AccessionQ9UHD0
GeneID29949
Source Escherichia coli.
Molecular Weight Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity 2µg/10µg/1000µg
AA Sequence LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/µg of rHuIL-19 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Reference1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13.
2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50.
3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97.
BackgroundHuman Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.