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货号:1005-01
Recombinant Streptavidin
rStreptavidin
技术参数
AccessionP22629
Source Escherichia coli.
Molecular Weight ~52,000 per tetramer.
Quantity 1mg/5mg/1g
AA Sequence MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
A282 of 0.1% solution 3.1
Purity > 98 % by SDS-PAGE and HPLC analyses
Specific Activity > 17 U/mg (one unit binds 1 µg D-biotin at pH 8.9)
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
Endotoxin Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.
Proteolytic Activity < 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
Reconstitution Dissolve with double distilled water
Clearly soluble in ddH2O (OD405nm < 0.1 at 10 mg/ml water) Storage (lyophilized)
SDS-PAGE